Mouse WISP1 ELISA Kit- Intracellular (RMEK-0154038)
Cat. No.: RMEK-0154038
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse WISP1 kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of WISP1 protein in Mouse cell lysates. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-2.2%; Inter-Assay-Extract-4-6.3% |
| Sensitivity | 10.517 pg/mL |
| Range | 31.25 pg/mL - 2000 pg/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-114-107% - 123% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 10.517 pg/mL; Range: 31.25 pg/mL - 2000 pg/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | WISP1 |
|---|---|
| UniProt ID | O95388 |
| Function | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
| Cellular Localization | Secreted |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
