Recombinant Human G-CSF Protein (Animal Free) (RMPP-00230602)
Cat. No.: RMPP-00230602
Category: Recombinant Protein
Research Area: Cardiovascular
INQUIRY
2 μg
1 mg
Product Features
| Source | HEK 293 cells |
|---|---|
| Purity | > 95% SDS-PAGE. |
| Nature | Recombinant |
| Endotoxin Level | < 1.000 Eu/µg |
| Animal Free | No |
| Tags | His tag N-Terminus |
| Form | Lyophilized |
| Applications | SDS-PAGE |
| Key Features | Expression system: HEK 293 cells; Purity: > 95% SDS-PAGE; Endotoxin level: < 1.000 Eu/µg; Tags: His tag N-Terminus; Suitable for: SDS-PAGE |
Protein Information
| UniProt ID | Q10589 |
|---|---|
| Molecular Weight | 15 kDa including tags |
| Molecular Weight Information | The recombinant human BST2 comprises 132 amino acids and has a predicted molecular mass of 14.9 kDa. The apparent molecular mass of the protein is approximately 23-26 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Sequence | NSEACRDGLR AVMECRNVTH LLQQELTEAQ KGFQDVEAQA ATCNHTVMAL MASLDAEKAQ GQKKVEELEG EITTLNHKLQ DASAEVERLR RENQVLSVRI ADKKYYPSSQ DS |
| Sequence Similarities | Belongs to the tetherin family. |
| Protein Length | Protein fragment |
| Cellular Localization | Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. |
| Tissue Specificity | Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). |
| Domain | The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. |
| Function | May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. |
| Post-translational Modifications | Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. |
Storage & Shipping
| Shipping and Storage | Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. pH: 7.4Constituent: 100% PBS |
|---|
For research use only. Not for clinical use.
