Recombinant Human Sonic Hedgehog Protein (Active) (RMPP-00230725)
Cat. No.: RMPP-00230725
Category: Recombinant Protein
Research Area: Stem Cells
INQUIRY
100 μg
Customer Size
Product Features
| Source | HEK 293 cells |
|---|---|
| Purity | > 96% SDS-PAGE. |
| Nature | Recombinant |
| Endotoxin Level | < 1.000 Eu/µg |
| Animal Free | No |
| Tags | His tag C-Terminus |
| Form | Lyophilized |
| Applications | SDS-PAGE; Functional Studies |
| Key Features | Expression system: HEK 293 cells; Purity: > 96% SDS-PAGE; Endotoxin level: < 1.000 Eu/µg; Active: Yes; Tags: His tag C-Terminus; Suitable for: SDS-PAGE, Functional Studies |
Protein Information
| UniProt ID | Q9BXY4 |
|---|---|
| Molecular Weight | 15 kDa including tags |
| Molecular Weight Information | The recombinant human RSPO3 consists of 136 amino acids and predicts a molecular mass of 15.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh RSPO3 is approximately 22-27 kDa due to different glycosylation. |
| Sequence | MHLRLISWLF IILNFMEYIG SQNASRGRRQ RRMHPNVSQG CQGGCATCSD YNGCLSCKPR LFFALERIGM KQIGVCLSSC PSGYYGTRYP DINKCTKCKA DCDTCFNKNF CTKCKSGFYL HLGKCLDNCP EGLEANNHTM ECVSIV |
| Sequence Similarities | Belongs to the R-spondin family. Contains 2 FU (furin-like) repeats. Contains 1 TSP type-1 domain. |
| Protein Length | Protein fragment |
| Cellular Localization | Secreted. |
| Tissue Specificity | Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node. |
| Domain | The FU repeats are required for activation and stabilization of beta-catenin. |
| Function | Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. |
Storage & Shipping
| Shipping and Storage | Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. pH: 7.40Constituent: 100% PBS This product is an active protein and may elicit a biological response in vivo, handle with caution. |
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For research use only. Not for clinical use.
