Animal-Free Recombinant Human IGF-II (RMPP-00231553)
Cat. No.: RMPP-00231553
Category: Growth Factors & Cytokines
INQUIRY
10 μg
50 μg
The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, altho μgh a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal thro μgh the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal thro μgh the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. The calculated molecular weight of Recombinant Human IGF-II is 7.5 kDa.
Product Features
| Source | E.coli |
|---|---|
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses. |
| Nature | Recombinant |
| Endotoxin Level | < 0.1 Eu/μg |
Protein Information
| UniProt ID | P01344 |
|---|---|
| Molecular Weight | 7.5 kDa |
| Molecular Weight Information | The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Sequence Similarities | Belongs to the insulin family. |
| Protein Length | Protein fragment |
| Cellular Localization | Secreted. |
| Function | The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. |
| Involvement in Disease | Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS). SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. |
| Post-translational Modifications | O-glycosylated with a core 1 or possibly core 8 glycan. |
Storage & Shipping
| Shipping and Storage | Shipped on Dry Ice. |
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For research use only. Not for clinical use.
