Organoid Culture Media Development Service

Organoid can simulate the three-dimensional structure and physiological functions of in vivo organs and tissues in vitro, and has a wide range of applications. Therefore, achieving high throughput and homogenization of high-quality culture of organoids in vitro is of great academic significance and application value to promote the comprehensive application of organoids. CD BioSciences has launched organoid media development services to advance the process of organoid applications and research.

Overview of Organoid Culture Media

Organoid culture requires a combination of basal medium with cytokines, amino acids, small molecule inhibitors, and other substances. The conditions vary based on tissue source. Many organoids are cultured in Matrigel, a mouse sarcoma-derived ECM-rich material, supplemented with growth factors. While Matrigel is versatile and economical, its complex composition (over 1800 proteins) complicates identification of signals needed for proper organoid formation. Moreover, it may lack essential components for certain organoids, and its mouse origin poses immunogenic risks for human clinical applications.

Methods of making organoids without Matrigel (Kozlowski, M.T., et al., 2021)

Organoid Medium Growth Factor Formulation

A key component of organoid media is the availability of essential growth factors. Typically, the production of growth factors relies on eukaryotic expression systems to ensure proper disulfide bond formation and macromolecular folding. In the eukaryotic expression system, growth factors are secreted from the expression host into the cell culture medium. This conditioned medium containing the selected growth factors is then diluted directly into organoid medium for application. The growth factors required for different organoid cultures are not exactly the same. For example, intestinal organoids require the addition of EGF, Noggin, R-Spondin-1, and Wnt-3a basic growth factors, whereas brain organoids require FGF- basic, Noggin, DKK-1, EGF, BDNF, and GDNF basic growth factors.

Microenvironment of cells (Kozlowski, M.T., et al., 2021)

Development of Organoid Culture Media

With the development of organoid culture technology, organoid culture media are also being innovated. Currently, the development of novel matrices such as decellularized extracellular matrix, synthetic hydrogels, gel-forming recombinant proteins, etc. provides new ideas for organoid culture solutions. Among them, decellularized methods have been proven to be suitable for the culture of human kidney, mouse kidney, mouse heart, human and porcine lung, and porcine testicular tissue. Synthetic hydrogels and gel-forming recombinant proteins have been characterized by mechanical properties, controllability of function and erosion rates, and programmability of MMP degradation enzyme recognition sites. Recombinant ECM proteins have been investigated for use in pancreatic-like organ cultures.

Our Services

CD BioSciences provides organoid culture media development services. Culture medium is an indispensable in vitro environment for organoid culture. It can provide nutritional factors, growth factors, and cell growth and development microenvironment for organoid culture in vitro. For this reason, we have launched our organoid culture media development service. Based on our professional laboratory team, we can work with you to solve your organoid culture related problems.

Custom Media Formulation: We offer tailored formulations designed to meet the unique growth conditions of your specific organoids. Each formulation is optimized for parameters like pH balance, nutrient composition, and growth factors.

Batch Production: Once the optimal media composition is finalized, we provide large-scale production services to ensure consistent quality for your ongoing projects.

Quality Testing: Rigorous testing protocols, including sterility checks and functional assays, guarantee that each batch of media meets the highest standards required for your organoid cultures.

Consultation and Optimization: Our scientific team works closely with you to understand your project goals, helping to fine-tune media conditions and provide ongoing support to maximize your experimental success.

As a pioneer in biotechnology, CD BioSciences has grown into one of the largest independent biotechnology companies in the world. CD BioSciences is committed to providing professional and efficient service to our customers around the world. If you are interested in our service, please contact us.