Liver Organoid Development Service
Liver organoids are able to replicate key aspects of human liver tissue, particularly the complex structure and metabolic functions. Researchers have now established liver organoids from multiple sources of induced pluripotent stem cells, embryonic stem cells, adult hepatocytes, and adult tissue-derived cells. The aim is to enrich one's toolbox and deepen one's understanding of liver biology and related diseases. CD BioSciences is now offering liver organoid development service.
Main Elements for Liver Organoid
Culture environment, signaling, and starting cell type play an integral role in liver organoid formation. The starting cell type determines the characteristics of the final liver-like organ, and different starting cells follow different pathways. Liver-like organs originate from monotypic cell cultures of pluripotent stem cells, adult stem cells, primary stem cells, and primary bile duct stem cells, or from multitype cell co-cultures of hepatic endodermal cells with MSCs and umbilical cord-derived endothelial cells. During liver-like organogenesis, different signals can stimulate or inhibit signaling pathways for organogenesis, thus directing the direction of pluripotent stem cell differentiation. The culture environment provides an organism-specific 3D extracellular matrix to support cell adhesion and growth.
Applications of Liver Organoids
The liver plays an important role in regulating energy balance and drug metabolism, and liver research has been a hot spot for research. Liver organoids can better mimic the metabolic functions of the liver, such as glycogen metabolism, lipid metabolism, steroid metabolism, drug metabolism, albumin secretion, and urea synthesis, and can highly express hepatocyte-associated drug metabolizing enzymes, nuclear receptors, and liver-specific markers.
Liver organoids that recapitulate adult tissue and liver regeneration
Early investigations by Michalopoulos and colleagues, in which isolated adult rat hepatocytes and other hepatic cells were placed in roller cultures, resulted in the creation of tissues that resembled characteristics of hepatic architecture, but these cultures only endured for a brief amount of time.
Self-renewing liver organoids demonstrating genetic stability during long-term culture were reported in 2013. Lgr5+ liver cells after damage induction or isolated healthy ducts self-organized into 3D structures that sustained long-term expansion as adult ductal progenitor cells while preserving the potential to develop into functional hepatocyte-like cells in vitro.
The use of an improved culture medium allowed adult ductal liver organoids to grow. The long-term cultivation of primary mouse hepatocytes, which maintain many morphological and functional characteristics of hepatocytes, has recently been documented by the Nusse and Clevers labs. Adult ductal organoids have also been produced from rats, cats, and dogs in addition to hepatic organoids from human and mouse cells.
Our Services
CD BioSciences offers liver organoid development services. The liver is an important target organ for drug toxicity, and it occupies an important position in biomedical research. For this purpose, we have introduced the following services. Based on our professional laboratory team, we can work with you to solve your liver organoid-related research problems.
As a pioneer in biotechnology, CD BioSciences has grown into one of the largest independent biotechnology companies in the world. CD BioSciences is committed to providing professional and efficient service to our customers around the world. If you are interested in our service,please contact us.
Reference
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Nicole Prior. et al. (2019). Liver organoids: from basic research to therapeutic applications. Gut. 68(12):2228-2237.
For research use only, not for clinical use.
