Cardiac Organoid Development Service
Cardiac organoids are the three-dimensional cellular structure similar to the human heart organ in vivo, and is a new generation of research on the model. Human cardiac organoids are composed of cells of different cardiac lineages derived from human pluripotent stem cells. This model accurately preserves the biological properties and functions of cells in vivo. CD BioSciences has launched a cardiac organoid development service to help people better explore the mysteries of human physiology.
Methods for Constructing Cardiac Rganoids
Human cardiac organoids are prepared by inducing aggregation of specific cells suspended in media or embedded in a 3D gel matrix. Physical and biochemical stimuli such as growth factors and electric fields provide important conditions for the organization and contraction of cardiac myocytes. These stimuli and the 3D membrane apparatus also further promote the formation of spatial organization of the cells, which eventually differentiate into microchambers of the beating human heart. Cardiac organoids hold great promise for observing the development of cardiac diseases, optimizing drug design, and assessing drug toxicity.
Recently, human pluripotent stem cell-derived embryoid bodies attached to collagen-bound hydrogels were found to be more efficient in forming myocardium-like tissue with branching vessels than those cultured in suspension. This result is because the collagen-bound hydrogel has a stiffness similar to that of myocardial tissue, which can promote cell proliferation and co-differentiation into cardiomyocytes.
Researchers prepared cardiac organoids using a 96-well machine with SU-8 photolithography and polydimethylsiloxane casting. This device allows for high-throughput screening, demonstrating that cardiac organoid models exhibit functional contractile tissue that resembles the biological properties of the natural heart.
To simulate biochemical and biophysical cues in vitro, a patterned micromold has been described for modeling the characteristics of early human cardiac organogenesis. This shape drove the spatial organization of human pluripotent stem cells and created the geometric constraints necessary to create the beating microchambers of the human heart during differentiation.
Generation, Applications and Challenges of Cardiac Organoids (Wanling Xuan. et al., 2019)
Challenges in Cardiac Organoids Development
Although some progress has been made in organoid research, multiple issues still need to be addressed in order to truly mimic the structure and function of in vivo tissues. For example, organoid cell differentiation and the construction of more sophisticated and complex organs. In addition, how to precisely regulate the combination of cytokines and the diffusion of nutrients in organoids are also key issues that need to be addressed.
Our Services
CD BioSciences offers cardiac organoid development services. Cardiac organoids are expected to be a tool for disease modeling and prediction of drug response, as well as a source for heart transplantation and cardiac development studies. For this purpose, we have launched our cardiac organoid development service. Based on our professional laboratory team, we can work with you to solve your cardiac organoid-related research problems.
As a pioneer in biotechnology, CD BioSciences has grown into one of the largest independent biotechnology companies in the world. CD BioSciences is committed to providing professional and efficient service to our customers around the world. If you are interested in our service,please contact us.
Reference
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Wanling Xuan. et al. (2019). Transformational Applications of Human Cardiac Organoids in Cardiovascular Diseases. Frontiers in Cell and Developmental Biology.
For research use only, not for clinical use.
