Intestinal Organoid Development Service

In recent years, intestinal stem cells have become the focus of research on intestinal injury regeneration, intestinal cancer and many other intestinal diseases. It has been shown that isolated intestinal stem cells can form intestinal organoids through 3D culture mode. Intestinal organoid is now widely used in human disease research due to its high culture success rate, short culture cycle, and small difference from the primary tissue. In this context, CD BioSciences offers intestinal organoid development service.

Overview of Intestinal Organoid

The intestinal organoid is a class of organs that includes all intestinal structures. They are generated from intestinal stem cells, extracted from adult tissues and cultured in three-dimensional medium. The intestinal organoids consist of a single layer of polarized intestinal epithelial cells surrounding a central lumen. By mimicking the crypt-villi structure of the intestine, its function, physiology, and organization, and retaining all cell types typically seen in this structure, a culture system for intestinal organoids was developed.

Development of Intestinal Organoids

Organoids can be derived from two sources of stem cell, organ-restricted adult stem cells (ASCs), and pluripotent stem cells (PSCs), both in the form of induced (iPSCs) and embryonic (ESCs). The formation and growth of organoids is critically depended on the culture medium components.

The key ingredients of the intestinal organoids culture medium include Wnt-3a (W), epidermal growth factor (EGF) (E), Noggin (N) and Rspondin 1 (R). Spatiotemporal addition of these growth factors into culture medium regulates the stem cell niche signaling pathways, including the Wnt, bone morphogenetic protein (BMP) and Notch, to induce ISCs self-renewal, proliferation and differentiation.

The introduction of additional combinations of small molecules can induce stem cell specific differentiation. Such as CHIR99021 and Valproic acid or LDN-193189 and CHIR99021, had a synergistic impact that promoted the maintenance of Lgr5+ ISCs in a self-renewal and undifferentiated state, leading to ISC-enriched cultures.

Supplementation of ENR medium with specific combinations of small molecules allows for differentiation phenotypes, such as DAPT and CHIR99021, Valproic acid and IWP-2, or DAPT and IWP-2.

Applications of Intestinal Organoids

• Intestinal Regeneration Studies
  Isolated intestinal stem cells are grown into intestinal organoids and can be kept functionally unchanged by prolonged culture disease. This could be an effective way to regenerate the intestine.

• Microbial Infection Studies
  Studies have shown that the intestinal organoid model has potential as a model for parasitic infection.

• Personalized Therapy 3D organoids derived from the patient's own cells are able to replicate very well some of the key characteristics of the primary tissue. They can therefore be used for high-throughput drug screening for a specific patient.

Our Services

CD BioSciences offers intestinal organoid development services. In addition to being used for intestinal regeneration, intestinal organoids are important models for studying intestinal nutrient transport, drug absorption, proliferative hormone secretion, and infection by various intestinal pathogens. For this purpose, we have launched our intestinal organoid development service. Based on our professional laboratory team, we can work with you to solve your intestinal organoid-related research problems.

As a pioneer in biotechnology, CD BioSciences has grown into one of the largest independent biotechnology companies in the world. CD BioSciences is committed to providing professional and efficient service to our customers around the world. If you are interested in our service,please contact us.

Reference

1. Sara Rahmani. et al. (2019). Intestinal Organoids: A New Paradigm for Engineering Intestinal Epithelium in vitro. Biomaterials.194:195-214.