Anti-68kDa Neurofilament/NF-L Antibody (RMAB-0250035)
Cat. No.: RMAB-0250035
Category: Primary Antibodies
INQUIRY
250 μL
1 mL
Mouse monoclonal to 68kDa Neurofilament/NF-L
Product Features
| Isotype | IgG1 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Clone Number | DA2 |
| Form | Liquid |
| Purity | Tissue culture supernatant |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length native protein (purified) corresponding to Pig 68kDa Neurofilament/NF-L. A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immunogen followed by IF microscopy. |
| Applications | Flow Cyt, ICC/IF, WB, IHC-P |
| Key Features | Mouse monoclonal to 68kDa Neurofilament/NF-L; Suitable for: Flow Cyt, ICC/IF, WB, IHC-P; Reacts with: Mouse, Rat, Human; Isotype: IgG1 |
Target Information
| Target Symbol | NEFL |
|---|---|
| Target Name | Neurofilament light polypeptide |
| UniProt ID | P07196 |
| Epitope | The epitope for this antibody is not in the N-terminus; however exactly where it is located is not known. It is thought to be somewhere within the central alpha helical "rod" region of the molecule. |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. |
| Involvement in Disease | Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F). CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E). CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. |
| Post-translational Modifications | O-glycosylated. Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. |
| Domain | The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. |
| Sequence Similarities | Belongs to the intermediate filament family. |
Storage & Shipping
| Storage Buffer | Preservative: 0.033% Sodium azide; Constituent: Tissue culture supernatant |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
