Anti-ADAM17 Antibody (RMAB-0250042)
Cat. No.: RMAB-0250042
Category: Primary Antibodies
INQUIRY
100 μL
10 µL
Rabbit monoclonal to ADAM17
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EPR21129-131 |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human |
| Immunogen | Recombinant fragment. |
| Applications | WB |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to ADAM17; Suitable for: WB; Knockout validated; Reacts with: Human |
Target Information
| Target Symbol | ADAM17 |
|---|---|
| Target Name | Disintegrin and metalloproteinase domain-containing protein 17 |
| UniProt ID | P78536 |
| Cellular Localization | Membrane. |
| Function | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway. |
| Post-translational Modifications | The precursor is cleaved by a furin endopeptidase. Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. |
| Domain | Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. |
| Sequence Similarities | Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. |
Storage & Shipping
| Storage Buffer | pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
