Anti-c-Myc (phospho T58) Antibody (RMAB-0250458)
Cat. No.: RMAB-0250458
Category: Primary Antibodies
INQUIRY
10 μL
100 μL
Rabbit monoclonal to c-Myc (phospho T58)
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EPR17923 |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide. |
| Applications | ICC/IF, WB, Flow Cyt (Intra), Dot blot |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to c-Myc (phospho T58); Suitable for: ICC/IF, WB, Flow Cyt (Intra), Dot blot; Reacts with: Human |
Target Information
| Target Symbol | MYC |
|---|---|
| Target Name | Myc proto-oncogene protein |
| UniProt ID | P01106 |
| Cellular Localization | Nucleus > nucleoplasm. Nucleus > nucleolus. |
| Function | Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. |
| Involvement in Disease | Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8; 12)(q24; q22) with BTG1.Defects in MYC are a cause of Burkitt lymphoma (BL). A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8; 14), t(8; 22) or t(2; 8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. |
| Post-translational Modifications | Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. |
| Sequence Similarities | Contains 1 basic helix-loop-helix (bHLH) domain. |
Storage & Shipping
| Storage Buffer | pH: 7.2; Preservative: 0.01% Sodium azide; Constituents: PBS, 40% Glycerol, 0.05% BSA |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
