Anti-CD59 Antibody (RMAB-0250388)
Cat. No.: RMAB-0250388
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Rabbit monoclonal to CD59
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EPR6425(2) |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide. |
| Applications | WB, IHC-P |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to CD59; Suitable for: WB, IHC-P; Knockout validated; Reacts with: Human |
Target Information
| Target Symbol | CD59 |
|---|---|
| Target Name | CD59 glycoprotein |
| UniProt ID | P13987 |
| Cellular Localization | Cell membrane. Secreted. Soluble form found in a number of tissues. |
| Function | Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. |
| Involvement in Disease | Defects in CD59 are the cause of CD59 deficiency (CD59D). |
| Post-translational Modifications | N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. |
| Sequence Similarities | Contains 1 UPAR/Ly6 domain. |
Storage & Shipping
| Storage Buffer | pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. |
For research use only. Not for clinical use.
