Anti-HDAC4 Antibody (RMAB-0250696)
Cat. No.: RMAB-0250696
Category: Primary Antibodies
INQUIRY
50 μL
Customer Size
Mouse monoclonal to HDAC4
Product Features
| Isotype | IgG2a |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Clone Number | HDAC-144 |
| Form | Liquid |
| Purity | Tissue culture supernatant |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide: MSSQSHPDGLSGRDQPVEL , corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. |
| Applications | WB |
| Key Features | Mouse monoclonal to HDAC4; Suitable for: WB; Knockout validated; Reacts with: Mouse, Rat, Human; Isotype: IgG2a |
Target Information
| Target Symbol | HDAC4 |
|---|---|
| Target Name | Histone deacetylase 4 |
| UniProt ID | P56524 |
| Cellular Localization | Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation. |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. |
| Involvement in Disease | Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR). A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism. |
| Post-translational Modifications | Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. |
| Domain | The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. |
| Sequence Similarities | Belongs to the histone deacetylase family. HD type 2 subfamily. |
Storage & Shipping
| Storage Buffer | pH: 7.40; Preservative: 0.0975% Sodium azide; Constituent: 0.0268% PBS |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
