Anti-Oct4 Antibody (RMAB-0250933)
Cat. No.: RMAB-0250933
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Rabbit monoclonal to Oct4
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EPR2054 |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide. |
| Applications | ChIC/CUT&RUN-seq, WB, IP, IHC-P |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to Oct4; Suitable for: ChIC/CUT&RUN-seq, WB, IP, IHC-P; Reacts with: Human |
Target Information
| Target Symbol | POU5F1 |
|---|---|
| Target Name | POU domain, class 5, transcription factor 1 |
| UniProt ID | Q01860 |
| Cellular Localization | Nucleus. Expressed in a diffuse and slightly punctuate pattern. |
| Developmental Stage | Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Post-translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. |
| Domain | The POU-specific domain mediates interaction with PKM2. |
| Sequence Similarities | Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. |
Storage & Shipping
| Storage Buffer | pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 40% Glycerol, 59% PBS, 0.05% BSA |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
