Anti-Oct4 Antibody (RMAB-0250934)
Cat. No.: RMAB-0250934
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Mouse monoclonal to Oct4
Product Features
| Isotype | IgG2b |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Clone Number | GT486 |
| Form | Liquid |
| Purity | Protein G purified |
| Species Reactivity | Human, Mouse |
| Immunogen | Recombinant fragment within Human Oct4 aa 1-182. The exact sequence is proprietary. |
| Applications | ICC/IF, Flow Cyt, IP, WB, IHC-P |
| Key Features | Mouse monoclonal to Oct4; Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P; Reacts with: Mouse, Human; Isotype: IgG2b |
Target Information
| Target Symbol | POU5F1 |
|---|---|
| Target Name | POU domain, class 5, transcription factor 1 |
| UniProt ID | Q01860 |
| Cellular Localization | Nucleus. Expressed in a diffuse and slightly punctuate pattern. |
| Developmental Stage | Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Post-translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. |
| Domain | The POU-specific domain mediates interaction with PKM2. |
| Sequence Similarities | Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. |
Storage & Shipping
| Storage Buffer | pH: 7.00; Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine) |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
