Anti-PDX1 Antibody (RMAB-0250963)
Cat. No.: RMAB-0250963
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Rabbit monoclonal to PDX1
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EPR3358(2) |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide within Human PDX1 (C terminal). The exact sequence is proprietary. |
| Applications | WB, IHC-P, ICC/IF |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to PDX1; Suitable for: WB, IHC-P, ICC/IF; Reacts with: Mouse, Human |
Target Information
| Target Symbol | PDX1 |
|---|---|
| Target Name | Pyruvate dehydrogenase protein X component, mitochondrial |
| UniProt ID | P52945 |
| Cellular Localization | Nucleus. |
| Function | Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. |
| Involvement in Disease | Defects in PDX1 are a cause of pancreatic agenesis (PAC). This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM); also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4); also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. |
| Post-translational Modifications | Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. |
| Domain | The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y). |
| Sequence Similarities | Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily. Contains 1 homeobox DNA-binding domain. |
Storage & Shipping
| Storage Buffer | pH: 7.2; Preservative: 0.01% Sodium azide; Constituents: 40% Glycerol, 0.05% BSA, 59% PBS |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
