Anti-SGK1 Antibody (RMAB-0251031)
Cat. No.: RMAB-0251031
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Rabbit monoclonal to SGK1
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | Y238 |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide. |
| Applications | Flow Cyt (Intra), WB, IHC-P |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to SGK1; Suitable for: Flow Cyt (Intra), WB, IHC-P; Reacts with: Mouse, Human |
Target Information
| Target Symbol | SGK1 |
|---|---|
| Target Name | Serine/threonine-protein kinase Sgk1 |
| UniProt ID | O00141 |
| Cellular Localization | Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation. |
| Function | Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1. |
| Post-translational Modifications | Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 12-amino acid. |
| Domain | Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 12 amino acids. |
| Sequence Similarities | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. |
Storage & Shipping
| Storage Buffer | pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA |
|---|---|
| Storage & Shipping | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
For research use only. Not for clinical use.
