Anti-YB1 Antibody (RMAB-0251214)
Cat. No.: RMAB-0251214
Category: Primary Antibodies
INQUIRY
10 μL
100 μL
Rabbit monoclonal to YB1
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EP2708Y |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide within Human YB1 aa 25 to the C-terminus |
| Applications | Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to YB1; Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P; Reacts with: Mouse, Rat, Human |
Target Information
| Target Symbol | YBX1 |
|---|---|
| Target Name | Y-box-binding protein 1 |
| UniProt ID | P67809 |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress. |
| Function | Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. |
| Post-translational Modifications | Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability. In the absence of phosphorylation the protein is retained in the cytoplasm. Cleaved by a 2S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus. |
| Sequence Similarities | Contains 1 CSD (cold-shock) domain. |
Storage & Shipping
| Storage Buffer | pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
