C166 (RMCL-00260309)
Cat. No.: RMCL-00260309
Category: Cell Lines
INQUIRY
1 vial
C166 is an endothelial cell line that was isolated from the yolk sac of a 12-day-old, mouse embryo. This cell line was deposited by R Auerbach and can be used in cardiovascular disease and stem cell research.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Cardiovascular disease research; Stem cell research |
| Specific Applications | The cell line should prove useful for the study of endothelial cell differentiation and for the determination of the mechanisms underlying the establishment of organ-specific endothelial cell heterogeneity. The cell line can support the stable proliferation of multipotential hematopoietic stem cells, thus generating adequate numbers of cells for study of the mechanisms involved in their subsequent development and differentiation. |
| Product Type | Animal cells |
| Organism | Mus musculus, mouse |
| Tissue | Yolk sac |
| Morphology | Endothelial |
| Cell Type | Endothelial Cell |
| Antigen Expression | Vascular cell adhesion molecule 1 (CD106, VCAM-1)RefWang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159vascular addressin +RefWang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159 |
| Genes Expressed | angiotensin converting enzyme (ACE) |
QC
| Mycoplasma Contamination | Not detected |
|---|
Doner Information
| Age | 12 days embryo |
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Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Handling procedure | To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial contents to a centrifuge tube containing 9. 0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes. Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7. 0 to 7. 6). Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. |
For research use only. Not for clinical use.
