Canine Cardiac Troponin I ELISA Kit- Extracellular (RMEK-0150557)
Cat. No.: RMEK-0150557
Category: ELISA Kits
INQUIRY
1 x 96 tests
Canine Cardiac Troponin I kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Dog |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Plasma-8-5.8%; Inter-Assay-Plasma-3-3.5% |
| Sensitivity | 0.368 ng/mL |
| Range | 1.172 ng/mL - 75 ng/mL |
| Sample Type | Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-88-86% - 91%; Cell culture media-113-107% - 118%; Hep Plasma-96-85% - 116%; EDTA Plasma-106-88% - 117%; Cit plasma-89-84% - 98% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.368 ng/mL; Range: 1.172 ng/mL - 75 ng/mL; Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Dog |
Target Information
| Target Symbol | TNNC1 |
|---|---|
| UniProt ID | P19429 |
| Biomarker of SCs/CSCs | Triple Negative Breast Cancer |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Involvement in Disease | Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1). RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
