CHO ACF Medium (RMMS-00560273)
Cat. No.: RMMS-00560273
Category: Specialized Medium
Research Area: Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing
INQUIRY
90 mL
Animal component-free, serum-free, glutamine-free semi-solid methylcellulose-based medium for selecting and cloning CHO cells
Product Features
| Overview | This product is a methylcellulose-based semi-solid medium with supplements for the selection and cloning of suspension-adapted CHO cells in serum-free conditions. This medium is animal component-free and contains only recombinant proteins and synthetic components. Single-cell cloning efficiency of CHO cells is higher in this medium than in protein-free media, especially at low plating densities. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthase (GS) selection systems. Benefits of semi-solid cloning: Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated. Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution. Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures. |
|---|---|
| Species | Other |
| Cell Type | CHO Cells |
| Applications | Cell Culture, Semi-Solid Cloning |
| Formulation | Animal Component-Free, Serum-Free |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
