Erythroid Kit (RMMS-00560247)
Cat. No.: RMMS-00560247
Category: Specialized Medium Kits
Research Area: Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology, Transplantation Research
INQUIRY
1 Kit
For differentiation of human ES or iPS cells to erythroblasts
Product Features
| Cell Type | Erythroid Cells, Hematopoietic Stem and Progenitor Cells |
|---|---|
| Formulation | Serum-Free |
| Species | Human |
| Applications | Cell Culture, Differentiation, Expansion |
| Description | Erythroid Kit is designed for the serum-free and feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to erythroid progenitor cells (erythroblasts) expressing Glycophorin A and CD71. This simple 2D differentiation protocol is performed in two stages. During the first 10- to 12-day stage, Hematopoietic Supplement A and then Supplement E1 are added to basal medium to induce cells toward erythroid-biased hematopoietic progenitor cells. At the end of this stage, hematopoietic progenitor cells are easily harvested from the culture supernatant. During the second 14-day stage, cells are further differentiated to erythroid progenitor cells using Supplement E2 and SFEM II (Day 10 - 24). The cells typically expand 241-fold +/- 190-fold (95% CI) during the 14-day erythroid differentiation stage and the Day 24 cell population typically contains 74% +/- 5% (95 CI) CD71+GlyA+ erythroblasts. Cells generated using Erythroid Kit can be further matured into normoblasts and reticulocytes when moved to appropriate culture conditions for maturation. Erythroid Kit has been optimized for differentiation of cells maintained in, and-. |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
