HFF-1 IRR (RMCL-00260304)
Cat. No.: RMCL-00260304
Category: Cell Lines
INQUIRY
1 vial
HFF-1 IRR is a fibroblast cell that was isolated from the foreskin of a donor. The irradiated cells can be used as feeder cells to support the growth of stem cells in the undifferentiated state.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Stem cell research |
| Specific Applications | Irradiated cells for use as feeder layer. These cells are provided to be used as feeder cells to support the growth of stem cells in the undifferentiated state. |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Skin; Foreskin |
| Morphology | Fibroblast |
| Cell Type | Fibroblast |
QC
| Mycoplasma Contamination | Not detected |
|---|
Doner Information
| Gender | Male |
|---|---|
| Age | Neonate |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | To insure the highest level of viability, be sure to warm media to 37°C before using it on the cells. It is not necessary to coat the culture vessel prior to thawing the cells. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Remove the vial from the water bath as soon as the contents are half way thawed (approximately 90 seconds), and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial’s contents plus 5 mL of complete growth medium to a 15 mL centrifuge tube. Use an additional 1 mL of media to rinse the vial and transfer the liquid to the 15 mL tube. Add 4 mL of complete growth media to bring the total volume to 10 mL. Gently mix and pellet the cells by centrifugation at 271 x g for 5 minutes. Discard the supernatant, resuspend the cells with fresh growth medium, and transfer to the appropriate size flask. Incubate 37°C in a 5% CO2 in air atmosphere. Fluid change twice a week or when pH decreases. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7. 0 to 7. 6). Medium Renewal: Twice a week or as pH decreases |
For research use only. Not for clinical use.
