Human ACE ELISA Kit(CD143) (RMEK-0152331)
Cat. No.: RMEK-0152331
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human ACE (CD143) in vitro ELISA kit is designed for the accurate quantitative measurement of Human ACE (CD143) in cell culture supernatants, serum, plasma (heparin) and saliva.
An ACE (CD143) specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for ACE (CD143) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human ACE (CD143) amount of sample captured in plate.
An ACE (CD143) specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for ACE (CD143) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human ACE (CD143) amount of sample captured in plate.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-1-16-= 4.9%; Intra-Assay-2-16-= 6.8%; Intra-Assay-3-16-= 4.5%; Inter-Assay-1-24-= 6.5%; Inter-Assay-2-24-= 7.6%; Inter-Assay-3-24-= 4.8% |
| Sensitivity | Sensitivity: 5 pg/mL |
| Range | 0.78 ng/mL - 50 ng/mL |
| Sample Type | Cell culture supernatant, Hep Plasma, Saliva, Serum |
| Key Features | Sensitivity: 5 pg/mL; Range: 0.78 ng/mL - 50 ng/mL; Sample type: Cell culture supernatant, Hep Plasma, Saliva, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | ACE |
|---|---|
| UniProt ID | P12821 |
| Biomarker of SCs/CSCs | Luminal A Breast Cancer |
| Function | Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. |
| Cellular Localization | Secreted and Cell membrane. |
| Post-transcriptional Modifications | Phosphorylated by CK2 on Ser-1299; which allows membrane retention. |
| Involvement in Disease | Ischemic stroke (ISCHSTR): A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Disease susceptibility is associated with variations affecting the gene represented in this entry. Renal tubular dysgenesis (RTD): Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). The disease is caused by mutations affecting the gene represented in this entry. Microvascular complications of diabetes 3 (MVCD3): Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Disease susceptibility is associated with variations affecting the gene represented in this entry. Intracerebral hemorrhage (ICH): A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Disease susceptibility is associated with variations affecting the gene represented in this entry. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
