Human Albumin ELISA Kit, Fluorescent (RMEK-0152349)
Cat. No.: RMEK-0152349
Category: ELISA Kits
INQUIRY
1 x 96 tests
Albumin in vitro ELISA kit is designed for the quantitative measurement of Albumin evels in human serum, plasma and cell culture supernatants. The ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Fluorescent |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-5.2%; Inter-Assay-Serum-3-7.6% |
| Sensitivity | 0.26 ng/mL |
| Range | 0.39 ng/mL - 100 ng/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-105-100% - 108%; Serum-100-92% - 110%; Hep Plasma-98-89% - 105%; EDTA Plasma-104-97% - 109%; Cit plasma-101-100% - 103% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.26 ng/mL; Range: 0.39 ng/mL - 100 ng/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Fluorescent; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | ALB |
|---|---|
| UniProt ID | P02768 |
| Biomarker of SCs/CSCs | Pancreatic Cancer |
| Function | Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.Glycated in diabetic patients.Phosphorylation sites are present in the extracelllular medium.Acetylated on Lys-223 by acetylsalicylic acid. |
| Involvement in Disease | Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH). FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
