Human Albumin ELISA Kit (RMEK-0152348)
Cat. No.: RMEK-0152348
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Albumin ELISA kit is a sandwich ELISA kit designed for the quantitative measurement of albumin levels in urine, saliva, milk, CSF, cell culture supernatants, cell lysate and tissue samples.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Albumin ELISA Kit from our range.
Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.
The concentration of the biotinylated antibody varies by lot: 30X, 40X, 50X, etc. The stock Biotinylated Albumin Antibody must be diluted with 1X Diluent N to prepare 1X Biotinylated Albumin Antibody for use in the assay procedure. Observe the label for the “X” concentration on the vial of Biotinylated Albumin Antibody.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Albumin ELISA Kit from our range.
Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.
The concentration of the biotinylated antibody varies by lot: 30X, 40X, 50X, etc. The stock Biotinylated Albumin Antibody must be diluted with 1X Diluent N to prepare 1X Biotinylated Albumin Antibody for use in the assay procedure. Observe the label for the “X” concentration on the vial of Biotinylated Albumin Antibody.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Time | 3h 00m |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall--5.9%; Inter-Assay-Overall--10.4% |
| Sensitivity | Sensitivity: 0.79 ng/mL |
| Range | 12.5 ng/mL - 100 ng/mL |
| Sample Type | Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Tissue, Urine |
| Key Features | Sensitivity: 0.79 ng/mL; Range: 12.5 ng/mL - 100 ng/mL; Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Tissue, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | ALB |
|---|---|
| UniProt ID | P02768 |
| Biomarker of SCs/CSCs | Pancreatic Cancer |
| Function | Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.Glycated in diabetic patients.Phosphorylation sites are present in the extracelllular medium.Acetylated on Lys-223 by acetylsalicylic acid. |
| Involvement in Disease | Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH). FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
