Human ALDH1A1 ELISA Kit (RMEK-0152350)
Cat. No.: RMEK-0152350
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human ALDH1A1 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALDH1A1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-A549 extract-8-4.8%; Inter-Assay-A549 extract-3-5.5% |
| Sensitivity | 220 pg/mL |
| Range | 1.25 ng/mL - 80 ng/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Serum-76-73% - 83%; Tissue Extracts-109-109% - 110%; Cell culture media-108-105% - 110%; Hep Plasma-75-70% - 80%; EDTA Plasma-86-78% - 96%; Cit plasma-74-68% - 80% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 220 pg/mL; Range: 1.25 ng/mL - 80 ng/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | ALDH1A1 |
|---|---|
| UniProt ID | P00352 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. |
| Cellular Localization | Cytoplasm. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
