Human BMP-4 ELISA Kit (RMEK-0152403)
Cat. No.: RMEK-0152403
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human BMP-4 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BMP-4 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-HT-29 S/N-5-6.3%; Inter-Assay-HT-29 S/N-3-4.9% |
| Sensitivity | 0.28 pg/mL |
| Range | 12 pg/mL - 750 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts |
| Recovery | Serum-89-86% - 91%; Cell culture extracts-104-97% - 108%; Tissue Extracts-92-87% - 99%; Cell culture media-118-110% - 125%; EDTA Plasma-85-80% - 90%; Cit plasma-87-82% - 93% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.28 pg/mL; Range: 12 pg/mL - 750 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | BMP4 |
|---|---|
| UniProt ID | P12644 |
| Biomarker of SCs/CSCs | Triple Negative Breast Cancer |
| Function | Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. |
| Cellular Localization | Secreted > extracellular space > extracellular matrix. |
| Involvement in Disease | Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6); also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies. Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11). Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
