Human BMP7 ELISA Kit (RMEK-0152404)
Cat. No.: RMEK-0152404
Category: ELISA Kits
INQUIRY
1 x 96 tests
BMP7 Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP7 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human BMP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman BMP7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Sensitivity | Sensitivity: 10 pg/mL |
| Range | 8.23 pg/mL - 6000 pg/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum |
| Recovery | Cell culture supernatant-97.11-84% - 105%; Serum-96.2-85% - 104%; Plasma-95.37-86% - 104% |
| Key Features | Sensitivity: 10 pg/mL; Range: 8.23 pg/mL - 6000 pg/mL; Sample type: Cell culture supernatant, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | BMP7 |
|---|---|
| UniProt ID | P18075 |
| Biomarker of SCs/CSCs | Skin Cancer |
| Function | Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A. Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, uses also BMPR2 as type II receptor. Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors. Promotes the expression of HAMP, this is repressed by its interaction with ERFE. |
| Cellular Localization | Secreted. |
| Development Stage | Expressed in the developing eye, brain and ear during embryogenesis. |
| Post-transcriptional Modifications | Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
