Human Bone Sialoprotein ELISA Kit (RMEK-0152420)
Cat. No.: RMEK-0152420
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Bone Sialoprotein ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Bone Sialoprotein in human serum and plsma. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-4.2%; Inter-Assay-Serum-3-7.3% |
| Sensitivity | 9 pg/mL |
| Range | 21.88 pg/mL - 1400 pg/mL |
| Sample Type | Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-106-104% - 107%; Hep Plasma-114-106% - 119%; EDTA Plasma-104-89% - 114%; Cit plasma-99-85% - 106% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 9 pg/mL; Range: 21.88 pg/mL - 1400 pg/mL; Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | BNSP |
|---|---|
| UniProt ID | P21815 |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.Sulfated on either Tyr-313 or Tyr-314. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
