Human BST2 ELISA Kit (RMEK-0152424)
Cat. No.: RMEK-0152424
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human BST2 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BST2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-9-2%; Inter-Assay-Serum-3-8.2% |
| Sensitivity | 0.28 pg/mL |
| Range | 5 pg/mL - 300 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-101-99% - 103%; Cell culture extracts-113-109% - 117%; Cell culture media-100-99% - 100%; Hep Plasma-96-93% - 1010%; EDTA Plasma-102-99% - 104%; Cit plasma-100-98% - 103% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.28 pg/mL; Range: 5 pg/mL - 300 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | BST2 |
|---|---|
| UniProt ID | Q10589 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. |
| Cellular Localization | Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. |
| Domain | The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. |
| Post-transcriptional Modifications | Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
