Human C8b (Complement C8b) ELISA Kit (RMEK-0152428)
Cat. No.: RMEK-0152428
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human C8b (Complement C8b) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human DystrophinH protein in human cell and tissue extract samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-3.2%; Inter-Assay-Serum-4-6.1% |
| Sensitivity | 0.1 ng/mL |
| Range | 0.391 ng/mL - 25 ng/mL |
| Sample Type | Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-113-111% - 116%; Cell culture media-92-86% - 95%; Hep Plasma-87-87% - 88%; EDTA Plasma-92-80% - 102%; Cit plasma-108-105% - 111% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.1 ng/mL; Range: 0.391 ng/mL - 25 ng/mL; Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | C8b |
|---|---|
| UniProt ID | P07358 |
| Function | Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | N-glycosylated; contains one or two bound glycans. Not O-glycosylated. |
| Involvement in Disease | Defects in C8B are a cause of complement component 8 deficiency type 2 (C8D2). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
