Human CD13 ELISA Kit (RMEK-0152457)
Cat. No.: RMEK-0152457
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD13 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD13 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-4.5% |
| Sensitivity | 25.6 pg/mL |
| Range | 219 pg/mL - 14000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-90%; Serum-110%; Hep Plasma-107%; EDTA Plasma-90%; Cit plasma-83% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 25.6 pg/mL; Range: 219 pg/mL - 14000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD13 |
|---|---|
| UniProt ID | P15144 |
| Function | Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. |
| Cellular Localization | Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. |
| Domain | Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). |
| Post-transcriptional Modifications | Sulfated.N- and O-glycosylated.May undergo proteolysis and give rise to a soluble form. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
