Human CD14 ELISA Kit (RMEK-0152460)
Cat. No.: RMEK-0152460
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD14 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD14 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. The technology. Quantitate Human CD14 with 4 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Human Serum-8-2.8%; Inter-Assay-Human Serum-3-2.1% |
| Sensitivity | 4 pg/mL |
| Range | 31.3 pg/mL - 2000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-102-95% - 106%; Serum-110-104% - 114%; Hep Plasma-109-106% - 111%; EDTA Plasma-110-105% - 115%; Cit plasma-109-99% - 116% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 4 pg/mL; Range: 31.3 pg/mL - 2000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD14 |
|---|---|
| UniProt ID | P08571 |
| Biomarker of SCs/CSCs | Triple Negative Breast Cancer |
| Function | Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. |
| Cellular Localization | Cell membrane. |
| Post-transcriptional Modifications | N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
