Human CD276 ELISA Kit (RMEK-0152464)
Cat. No.: RMEK-0152464
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD276 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD276 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 2h 30m |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall-3-2.9%; Inter-Assay-Overall-5-9.9% |
| Sensitivity | Sensitivity: 178 pg/mL |
| Range | 578 pg/mL - 37000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine |
| Recovery | Cell culture supernatant-94-83% - 101%; Urine-89-88% - 91%; Serum-102-100% - 104%; Hep Plasma-87-85% - 89%; EDTA Plasma-91-87% - 94%; Cit plasma-94-90% - 100% |
| Key Features | Sensitivity: 178 pg/mL; Range: 578 pg/mL - 37000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD276 |
|---|---|
| UniProt ID | Q5ZPR3 |
| Function | May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. |
| Cellular Localization | Membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
