Human CD33 ELISA Kit (RMEK-0152467)
Cat. No.: RMEK-0152467
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD33 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD33 protein in human serum, plasma and cell culture extract samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-3.1%; Inter-Assay-Serum-3-5.1% |
| Sensitivity | 11.39 pg/mL |
| Range | 70.31 pg/mL - 4500 pg/mL |
| Sample Type | Cell Lysate, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-98-97% - 101%; Cell Lysate-93-86% - 98%; Hep Plasma-89-89% - 90%; EDTA Plasma-91-90% - 92%; Cit plasma-88-83% - 93% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 11.39 pg/mL; Range: 70.31 pg/mL - 4500 pg/mL; Sample type: Cell Lysate, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD33 |
|---|---|
| UniProt ID | P20138 |
| Function | Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). |
| Cellular Localization | Cell membrane. |
| Domain | Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. |
| Post-transcriptional Modifications | Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
