Human CD4 ELISA Kit (RMEK-0152469)
Cat. No.: RMEK-0152469
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD4 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD4 protein in cell culture extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Cell extract-3-1.2%; Inter-Assay-Cell extract-8-6.4% |
| Sensitivity | 13.7 pg/mL |
| Range | 0.469 ng/mL - 30 ng/mL |
| Sample Type | Cell culture extracts |
| Recovery | Cell culture extracts-100-96% - 105% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 13.7 pg/mL; Range: 0.469 ng/mL - 30 ng/mL; Sample type: Cell culture extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD4 |
|---|---|
| UniProt ID | P01730 |
| Biomarker of SCs/CSCs | Dukes Type C Colon Cancer |
| Function | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
| Cellular Localization | Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. |
| Post-transcriptional Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
