Human CD44 ELISA Kit (RMEK-0152471)
Cat. No.: RMEK-0152471
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD44 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD44 protein in human serum, plasma, and tissue extract samples. The technology. Quantitate Human CD44 with 12. 28 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-5.5%; Inter-Assay-Serum-3-6.9% |
| Sensitivity | 12.28 pg/mL |
| Range | 62.5 pg/mL - 4000 pg/mL |
| Sample Type | Cell Lysate, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-111-107% - 113%; Cell Lysate-112-103% - 117%; Hep Plasma-107-104% - 114%; EDTA Plasma-104-99% - 108%; Cit plasma-112-100% - 120% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 12.28 pg/mL; Range: 62.5 pg/mL - 4000 pg/mL; Sample type: Cell Lysate, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD44 |
|---|---|
| UniProt ID | P16070 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. |
| Cellular Localization | Membrane. |
| Domain | The lectin-like LINK domain is responsible for hyaluronan binding. |
| Post-transcriptional Modifications | Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.N-glycosylated.O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
