Human CD59 ELISA Kit (RMEK-0152476)
Cat. No.: RMEK-0152476
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD59 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD59 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-7.5%; Inter-Assay-Serum-3-6.4% |
| Sensitivity | 4.9 pg/mL |
| Range | 23.44 pg/mL - 1500 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine |
| Recovery | Cell culture supernatant-112-107% - 116%; Urine-111-107% - 120%; Serum-90-82% - 95%; Tissue Extracts-99-93% - 109%; Hep Plasma-100-84% - 109%; EDTA Plasma-92-85% - 97%; Cit plasma-94-80% - 117% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 4.9 pg/mL; Range: 23.44 pg/mL - 1500 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD59 |
|---|---|
| UniProt ID | P13987 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. |
| Cellular Localization | Cell membrane. Secreted. Soluble form found in a number of tissues. |
| Post-transcriptional Modifications | N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. |
| Involvement in Disease | Defects in CD59 are the cause of CD59 deficiency (CD59D). |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
