Human CD69 ELISA Kit (RMEK-0152481)
Cat. No.: RMEK-0152481
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD69 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurofilament H protein in serum, plasma, cell culture supernatant and cell extract samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-3.3%; Inter-Assay-Extract-3-3.4% |
| Sensitivity | 25.97 pg/mL |
| Range | 93.75 pg/mL - 6000 pg/mL |
| Sample Type | Cell culture media, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Serum-103-100% - 106%; Tissue Extracts-108-102% - 115%; Cell culture media-85-81% - 91%; Hep Plasma-98-86% - 110%; EDTA Plasma-90-86% - 93%; Cit plasma-110-99% - 116% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 25.97 pg/mL; Range: 93.75 pg/mL - 6000 pg/mL; Sample type: Cell culture media, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD69 |
|---|---|
| UniProt ID | Q07108 |
| Function | Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. |
| Cellular Localization | Membrane. |
| Development Stage | Earliest inducible cell surface glycoprotein acquired during lymphoid activation. |
| Post-transcriptional Modifications | Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
