Human CD70 ELISA Kit (RMEK-0152483)
Cat. No.: RMEK-0152483
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD70 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD70 protein in cell lysate and cell culture extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-7.3%; Inter-Assay-Extract-3-8% |
| Sensitivity | 0.1 ng/mL |
| Range | 0.28 ng/mL - 18 ng/mL |
| Sample Type | Cell culture extracts, Cell Lysate |
| Recovery | Cell culture extracts-120-116% - 123%; Cell Lysate-123-121% - 126% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.1 ng/mL; Range: 0.28 ng/mL - 18 ng/mL; Sample type: Cell culture extracts, Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD70 |
|---|---|
| UniProt ID | P32970 |
| Biomarker of SCs/CSCs | Luminal A Breast Cancer |
| Function | Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. |
| Cellular Localization | Membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
