Human CD80 ELISA Kit(B7-1) (RMEK-0152484)
Cat. No.: RMEK-0152484
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD80 ELISA Kit (B7-1) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD80 (B7-1) protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Cow, Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-4.9%; Inter-Assay-Serum-3-6.5% |
| Sensitivity | 31 pg/mL |
| Range | 78 pg/mL - 5000 pg/mL |
| Sample Type | Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-110-106% - 113%; Serum-101-99% - 105%; Cell culture media-97-95% - 100%; Hep Plasma-92-88% - 98%; EDTA Plasma-97-94% - 101%; Cit plasma-103-99% - 109% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 31 pg/mL; Range: 78 pg/mL - 5000 pg/mL; Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Cow, Human |
Target Information
| Target Symbol | CD80 |
|---|---|
| UniProt ID | P33681 |
| Function | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. |
| Cellular Localization | Membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
