Human CD86 ELISA Kit (RMEK-0152485)
Cat. No.: RMEK-0152485
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD86 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD86 protein in cell culture supernatant. The technology. Quantitate Human CD86 with 3. 5 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Protein-8-2% |
| Sensitivity | 3.5 pg/mL |
| Range | 15.63 pg/mL - 1000 pg/mL |
| Sample Type | Cell culture supernatant |
| Recovery | Cell culture supernatant-98% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 3.5 pg/mL; Range: 15.63 pg/mL - 1000 pg/mL; Sample type: Cell culture supernatant; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD86 |
|---|---|
| UniProt ID | P42081 |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. |
| Cellular Localization | Membrane. |
| Post-transcriptional Modifications | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
