Human CNTF ELISA Kit (RMEK-0152520)
Cat. No.: RMEK-0152520
Category: ELISA Kits
INQUIRY
1 x 96 tests
CNTF Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in plasma and cell culture supernatants. (Human CNTF concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Sensitivity | Sensitivity: 0.2 ng/mL |
| Range | 0.823 ng/mL - 200 ng/mL |
| Sample Type | Cell culture supernatant, Plasma |
| Recovery | Cell culture supernatant-95.45-84% - 104%; Plasma-93.28-83% - 104% |
| Key Features | Sensitivity: 0.2 ng/mL; Range: 0.823 ng/mL - 200 ng/mL; Sample type: Cell culture supernatant, Plasma; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CNTF |
|---|---|
| UniProt ID | P26441 |
| Function | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. |
| Cellular Localization | Cytoplasm. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
