Human COMP ELISA Kit (RMEK-0152527)
Cat. No.: RMEK-0152527
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human COMP ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human COMP protein in human serum, plasma, and cell culture supernatants samples. The technology. Quantitate Human COMP with 116 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Plasma-8-4.5%; Inter-Assay-Plasma-3-5.4% |
| Sensitivity | 116 pg/mL |
| Range | 781.25 pg/mL - 50000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Serum |
| Recovery | Cell culture supernatant-106-94% - 115%; Serum-104-102% - 105%; EDTA Plasma-117-114% - 118%; Cit plasma-115-113% - 116% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 116 pg/mL; Range: 781.25 pg/mL - 50000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | COMP |
|---|---|
| UniProt ID | P49747 |
| Function | May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. |
| Cellular Localization | Secreted > extracellular space > extracellular matrix. |
| Development Stage | Present during the earliest stages of limb maturation and is later found in regions where the joints develop. |
| Domain | The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.The TSP C-terminal domain mediates interaction with FN1 and ACAN. |
| Involvement in Disease | Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
