Human CREBBP ELISA Kit (RMEK-0152535)
Cat. No.: RMEK-0152535
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CREBBP kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CREBBP protein in Cell Lysate, Tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-4.6%; Inter-Assay-Extract-3-11.3% |
| Sensitivity | 27.732 pg/mL |
| Range | 70.313 pg/mL - 4500 pg/mL |
| Sample Type | Cell Lysate, Tissue Extracts |
| Recovery | Tissue Extracts-100-90% - 115%; Cell Lysate-104-92% - 111% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 27.732 pg/mL; Range: 70.313 pg/mL - 4500 pg/mL; Sample type: Cell Lysate, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CREBBP |
|---|---|
| UniProt ID | Q92793 |
| Biomarker of SCs/CSCs | Pancreatic Cancer |
| Function | Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. |
| Cellular Localization | Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. |
| Domain | The KIX domain mediates binding to HIV-1 Tat. |
| Post-transcriptional Modifications | Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.Phosphorylated upon DNA damage, probably by ATM or ATR.Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX. |
| Involvement in Disease | Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8; 16)(p11; p13) with MYST3/MOZ; translocation t(11; 16)(q23; p13. 3) with MLL/HRX; translocation t(10; 16)(q22; p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription. Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1). RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
