Human DKK3 ELISA Kit(Dickkopf-3) (RMEK-0152562)
Cat. No.: RMEK-0152562
Category: ELISA Kits
INQUIRY
1 x 96 tests
DKK3 (Dickkopf-3) Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK3 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human DKK3 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Sensitivity | Sensitivity: 3 pg/mL |
| Range | 2.74 pg/mL - 2000 pg/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum |
| Recovery | Cell culture supernatant-96.23-85% - 105%; Serum-86.69-77% - 96%; Plasma-89.24-80% - 98% |
| Key Features | Sensitivity: 3 pg/mL; Range: 2.74 pg/mL - 2000 pg/mL; Sample type: Cell culture supernatant, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | DKK3 |
|---|---|
| UniProt ID | Q9UBP4 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). |
| Cellular Localization | Secreted. |
| Domain | The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. |
| Post-transcriptional Modifications | N- and O-glycosylated. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
