Human DMP1 ELISA Kit (RMEK-0152565)
Cat. No.: RMEK-0152565
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human DMP1 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DMP1 protein in cell culture supernatant, cit plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Supernatant-5-6.6%; Inter-Assay-Supernatant-3-6.7% |
| Sensitivity | 22.58 pg/mL |
| Range | 171.875 pg/mL - 11000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, Serum |
| Recovery | Cell culture supernatant-108-93% - 120%; Serum-83-82% - 84%; Cit plasma-84-83% - 86% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 22.58 pg/mL; Range: 171.875 pg/mL - 11000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | DMP1 |
|---|---|
| UniProt ID | Q13316 |
| Biomarker of SCs/CSCs | Luminal A Breast Cancer |
| Function | May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. |
| Cellular Localization | Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus. |
| Post-transcriptional Modifications | Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. |
| Involvement in Disease | Hypophosphatemic rickets, autosomal recessive, 1 (ARHR1): A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities. The disease is caused by variants affecting the gene represented in this entry. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
