Human EHMT2/G9A ELISA Kit (RMEK-0152575)
Cat. No.: RMEK-0152575
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human EHMT2/G9A kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human EHMT2/G9A protein in cell extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-6.9%; Inter-Assay-Extract-3-11.1% |
| Sensitivity | 3.065 pg/mL |
| Range | 15.625 pg/mL - 1000 pg/mL |
| Sample Type | Cell culture extracts |
| Recovery | Cell culture extracts-93-84% - 109% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 3.065 pg/mL; Range: 15.625 pg/mL - 1000 pg/mL; Sample type: Cell culture extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | G9A |
|---|---|
| UniProt ID | Q96KQ7 |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| Cellular Localization | Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. |
| Domain | The SET domain mediates interaction with WIZ.The ANK repeats bind H3K9me1 and H3K9me2. |
| Post-transcriptional Modifications | Methylated at Lys-185; automethylated. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
