Human Erythropoietin ELISA Kit(EPO) (RMEK-0152584)
Cat. No.: RMEK-0152584
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Erythropoietin ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Erythropoietin protein in serum, plasma and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-6.1%; Inter-Assay-Serum-3-10% |
| Sensitivity | 1 mIU/mL |
| Range | 3.125 mIU/mL - 200 mIU/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-112-109% - 114%; Serum-99-98% - 100%; Hep Plasma-91-90% - 92%; EDTA Plasma-104-96% - 112%; Cit plasma-98-86% - 111% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 1 mIU/mL; Range: 3.125 mIU/mL - 200 mIU/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | EPO |
|---|---|
| UniProt ID | P01588 |
| Biomarker of SCs/CSCs | Hepatic Cancer |
| Function | Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. |
| Cellular Localization | Secreted. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
